Kinetic parametersa of NADPH and NADH diaphorase reactions that were catalyzed by Xac-FNR, pea-FNR and Ec-FNR, and the dissociation constants for the different complexes with NADP+ b.

aEach parameter value represents the average of three independent determinations. A description of the calculation methods that were employed is reported in the Materials and Methods. The original data are depicted in Figure S2.
bPotassium ferricyanide reduction was assessed using the diaphorase assay of Zanetti [41] in 50 mM Tris-HCl (pH 8.0) using NADPH or NADH as the substrate.
cValues of parameters for pea-FNR and Ec-FNR were obtained from a reference [49].
dAn estimate of the limit of the determination based on the tested sensitivity of the method.
eNot determined.