Membrane proteins follow multiple pathways to the basolateral cell surface in polarized epithelial cells
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https://www.omicsdi.org/dataset/bioimages/S-JCBD-200901021
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SNAP-cells were transfected with a plasmid encoding Ts 045 VSV-G-YFP and incubated for 24h at 40 degrees to accumulate newly synthesized VSV-G in the ER. Next, samples were BG-Blocked, washed and incubated for a short 10 minute recovery period to permit synthesis of new SNAP tagged sodium pump protein. Samples were subjected to 19 degree Golgi block and fixed immediately. Samples were labeled with TMR-STAR (red) and imaged for YFP (green).
创建时间：
2018-11-29
