The Effects of Different Forms of Zeatin on Prepulse Inhibition and Anxiety

In this study, behavioral responses to various doses of trans-zeatin (tZ) and trans-zeatin riboside (tZR) on prepulse inhibition following sleep deprivation and anxiety in 12-14 week old Wistar Albino rats (n=128) were examined.

In sleep deprivation experiments, prepulse inhibition test and locomotor activity test were performed to 8 groups of rats (n=64) that were deprived of sleep for 72 hours using the modified multiple platform technique, following administration of vehicle or tZR intraperitoneally at doses of 2.5-5-10-20 mg/kg or 2.5-5-10 mg/kg, respectively. The groups are as follows: 1) SD control (vehicle) group, 2) SD 2,5 mg/kg tZ group, 3) SD 5 mg/kg tZ group, 4) SD 10 mg/kg tZ group, 5) SD 20 mg/kg tZ group, 6) SD 2,5 mg/kg tZR group, 7) SD 5 mg/kg tZR group, 8) SD 10 mg/kg tZR group. All.data.part.1 excel file shows the data of all groups for basal mean PPI values, mean PPI values in 74/78/86 db prepulse stimuli respectively, startle values, weights, and locomotor activity values (at 10 min time bins and total).

In anxiety experiments, the elevated plus maze test was applied to 8 groups of rats (n=64) who were administered intraperitoneally with vehicle or tZ at doses of 2.5-5-10 mg/kg, or tZR at doses of 2.5-5-10-20 mg/kg, respectively. The groups are as follows: 1) Control (vehicle) group, 2) 2,5 mg/kg tZ group, 3) 5 mg/kg tZ group, 4) 10 mg/kg tZ group, 5) 2,5 mg/kg tZR group, 6) 5 mg/kg tZR group, 7) 10 mg/kg tZR group, 8) 20 mg/kg tZR group. All.data.part.2 excel file shows the data of all groups for entries into closed arm (EICA), entries into open arm (EIOA), time spent in closed arm (TSICA), time spent in open arm (TSIOA), entries into closed arm percentage (EICA%), entries into open arm percentage (EIOA%), time spent in closed arm percentage (TSICA%), time spent in open arm percentage (TSIOA%) , and weights.

It was found in the study that the doses of tZ 5 mg/kg and tZR 20 mg/kg had anxiogenic effects in anxiety experiments. In sleep deprivation experiments, however, tZ and tZR did not reverse impaired prepulse inhibition at any dose, and did not show a significant effect on startle levels and locomotor activity. These results indicate that tZ and tZR do not have a therapeutic effect on the psychosis model triggered by sleep deprivation in rats, and do not show anxiolytic effects, but may show anxiogenic effects on the contrary.

本研究旨在探讨不同剂量反式赤藓糖醇（tZ）及其核苷酸（tZR）对12-14周龄Wistar白化大鼠（n=128）在睡眠剥夺及焦虑状态下的前脉冲抑制行为反应的影响。在睡眠剥夺实验中，采用改良的多平台技术对睡眠剥夺72小时的8组大鼠（n=64）进行了前脉冲抑制测试和运动活动测试，并在腹腔内分别注射溶剂或tZR，剂量分别为2.5-5-10-20 mg/kg或2.5-5-10 mg/kg。各组别如下：1）睡眠剥夺对照组（溶剂），2）睡眠剥夺2.5 mg/kg tZ组，3）睡眠剥夺5 mg/kg tZ组，4）睡眠剥夺10 mg/kg tZ组，5）睡眠剥夺20 mg/kg tZ组，6）睡眠剥夺2.5 mg/kg tZR组，7）睡眠剥夺5 mg/kg tZR组，8）睡眠剥夺10 mg/kg tZR组。所有数据的第一部分Excel文件显示了各组基础平均前脉冲抑制值、不同强度前脉冲刺激下的平均前脉冲抑制值、惊跳值、体重以及运动活动值（以10分钟的时间间隔和总运动活动量）。在焦虑实验中，对腹腔内注射溶剂或tZ（剂量分别为2.5-5-10 mg/kg）或tZR（剂量分别为2.5-5-10-20 mg/kg）的8组大鼠（n=64）进行了 Elevated Plus Maze 测试。各组别如下：1）对照组（溶剂），2）2.5 mg/kg tZ组，3）5 mg/kg tZ组，4）10 mg/kg tZ组，5）2.5 mg/kg tZR组，6）5 mg/kg tZR组，7）10 mg/kg tZR组，8）20 mg/kg tZR组。所有数据的第二部分Excel文件展示了各组进入封闭臂（EICA）、进入开放臂（EIOA）、在封闭臂停留时间（TSICA）、在开放臂停留时间（TSIOA）、进入封闭臂百分比（EICA%）、进入开放臂百分比（EIOA%）、在封闭臂停留百分比（TSICA%）和开放臂停留百分比（TSIOA%）以及体重数据。研究发现，在焦虑实验中，tZ 5 mg/kg和tZR 20 mg/kg剂量表现出焦虑诱导效应。然而，在睡眠剥夺实验中，无论剂量如何，tZ和tZR均未能逆转受损的前脉冲抑制，且对惊跳水平和运动活动没有显著影响。这些结果表明，tZ和tZR对由睡眠剥夺引起的大鼠精神分裂模型不具有治疗作用，且未显示出抗焦虑效果，反而可能表现出焦虑诱导效应。