GEP signatures of primary breast cells radiation treated.

Breast cancer (BC) recovery has considerably increased thanks to the advances achieved from research in this field. However, despite the important results obtained, BC remains a complex multifactorial disease with distinct subtypes associated with different outcomes.So, many efforts from radiobiological research are needed to help clinicians in understanding molecular features of a specific tumor subtype, in order to better define the most successful treatment plan, including the choice of the best Radiation Teraphy (RT) modality and schedule in clinical practice. The aim of the present study was to analyze the GEPs in primary breast cancer cells following irradiation with doses of 9 Gy and 23 Gy electron beam delivered by IOERT treatment, in order to define gene signatures of response to high doses of IR. Radiation-induced gene expression changes in healthy (HMEC) and BC (BCpc7 and BCpcEMT) primary cultures obtained from surgical biopsies, were analyzed as two-color hybridizations using Agilent Technologies whole human genome 4x44K microarrays. Samples were named as follow: ; HMEC_9Gy; HMEC_23Gy; BCpc7_9Gy; BCpc7_23Gy, BCpcEMT_9Gy, BCpcEMT_23Gy.