Microscopy data for 'The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosis'

DNA double-strand breaks are dangerous lesions in mitosis as they can lead to mis-segregation of acentric chromosome fragments. We previously showed that MDC1 and TOPBP1 are critically implicated in the maintenance of chromosomal stability during mitosis. In this study we reveal that the CIP2A protein is mediating TOPBP1 accumulation at sites of DNA breaks in mitosis. Furthermore, we show that CIP2A deficient cells are sensitive to DNA breaks and chromosmally unstable.