Pangenome gene alignments of 41 Pseudorhizobium and Neorhizobium strains
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All predicted protein sequence were extracted from the 571Rhizob genome dataset and clustered into homologous gene families (with possibly multiple copies per genome) using MMSeqs2 release 2 (commit e5d64b2) (Steinegger & Söding 2017). Protein sequences from each gene family were aligned using ClustalOmega (Sievers et al. 2011) and reverse-translated into coding sequence (CDS) alignments using PAL2NAL (Suyama et al. 2006).This resulted in the extensive Dataset presented in https://doi.org/10.6084/m9.figshare.8343473. Alignment file set and sequence content were then restricted to sequences occurring in the the 41NeoPseudo sub-dataset, resulting into 6,714 gene family alignments.
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figshare创建时间：
2019-06-27
