Comparative RNA seq analysis of Salmonella enterica serovar Typhimurium and a pan methionine sulfoxide reductase gene deletion (?5msr mutant) strain

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major foodborne pathogen which suffers but survives oxidative stress. Methionine sulfoxide reductases (Msrs) are important antioxidant enzymes that protect cells from oxidative damage by reducing oxidized methionine residues (free) or bound in proteins, back to methionine. To examine the global transcriptional consequences of deleting all five known msr genes, RNA sequencing was performed on S. Typhimurium and ?5msr mutant strains. This dataset provides a comprehensive transcriptomic resource for investigating the impact of msrs deletion on oxidative stress–associated regulatory pathways and cellular processes in S. Typhimurium. Overall design: S. Typhimurium and ?5msr mutant strains were grown to mid-logarithmic phase (OD600 ˜ 1.0). Total RNA was extracted, ribosomal RNA was depleted, and strand-specific RNA-seq libraries were prepared and sequenced on an Illumina platform. Sequencing reads were quality filtered, rRNA reads were removed, and the remaining reads were aligned to the Salmonella Typhimurium LT2 reference genome (GCF_000006945.2). Gene-level read counts were generated and normalized for comparative analysis of gene expression between the two strains.