Additional file 2 of Application of gold immunochromatographic assay strip combined with digital evaluation for early detection of Toxoplasma gondii infection in multiple species

Additional file 2: Figure S1. Optimum pH and conjugate concentrations of mouse IgG. (a) The colloidal gold solution was adjusted to different pH values using 0.2 M K2CO3 to obtain an optimum pH value for mouse IgG. (b) A twofold series showing an increasing amount of mouse IgG added to a colloidal gold solution to identify the optimum conjugate amount of murine protein. Figure S2. Stability of the AMA1C-GICA strips. P: Toxoplasma gondii-positive control; N: T. gondii-negative control; PBS: blank control. Figure S3. Inter- and intra-batch variation of the AMA1C-GICA strips. P: Toxoplasma gondii-positive control, N: T. gondii-negative control.