BMAL1 represses transposable elements independently of CLOCK in pluripotent cells [Hi-C]

Genome profiling of BMAL1,TRIM28 and H3K9me3 analyzed by ChIP-seq in JM8+/+ and Bmal1-/- mESCs. Genome profiling of FlagBMAL1 in mESCs. High throughput capture chromosome conformation analysis (Hi-C) in JM8+/+ and Bmal1-/- mESCs. mRNA-seq analysis of JM8+/+ and Clock-/- mESCs Overall design: In situ Hi-C (High-throughput Chromosome Conformation Capture) assays were performed in two biological replicates of mutant and wild-type cells to assess the 3D genome structure changes upon BMAL1 depletion