Effect of SGC0946 and SGC0649 on CD4+ T cells under Th1 polarizing conditions

Comparison between naïve CD4+ mouse T cells and either bona fide IFNg+ and IFNg- CD4+ T cells after culturing under Th1 polarizing conditions in the presence of either the epigenetic probe SGC0946 or SGC0649 Overall design: 3 naïve biological replicates (CD4+ CD44- CD62L+), 3 biological replicates of enriched CD4+ T cells that were polarized for 4 days under Th1 polarizing conditions and FACS sorted for bona fide IFN-g+ (YFP-reporter+) or IFN-g- (YFP-reporter-) cells in the presence of 1 µM of either SGC0946 or SGC0649