Generation of Th1/Th17 mediated immunity in response to C.difficile in an in-vitro murine model system.

WT BMDCs were infected with PFA-fixed C. difficile strains. 24 h post-infection, stimulated BMDCs were co-cultured with WT splenocytes in the presence of anti-CD3/CD28. 96 h post co-culture, secreted cytokines IFN-γ (A), IL-17A (B), IL-10 (C), IL-4 (D) and IL-2 (E) were measured by ELISA. 1 µg/ml LPS stimulation served as reference control. Data represent mean ± SEM, n = 3. */∧p∧∧pP values were obtained using ANOVA with Bonferroni post-test analysis.