File S1 - Inheritance of Hetero-Diploid Pollen S-Haplotype in Self-Compatible Tetraploid Chinese Cherry (Prunus pseudocerasus Lindl)

Supporting information tables and figures. Table S1 Sequences of primers used in this study. Table S2 Rates of fruit setting in self- or cross-pollination of four cultivars. Table S3 Gamete constitutions in the interspecific cross-pollinated progeny of diploid with hetero-tetraploid plants. Table S4 Gamete constitutions in the self-pollinated progeny of hetero-tetraploid plants. Figure S1 Digestion patterns of PCR amplification products of SFBs with six different restriction endonucleases for “Dabai”. M. Ladder marker; 1–23. Digestion patterns of twenty-three independent positive clones. a. Digestion patterns of PCR amplification products with restriction endonuclease DpnII; b. Digestion patterns of PCR amplification products with restriction endonuclease Csp6I; c. Digestion patterns of PCR amplification products with restriction endonuclease HinfI; d. Digestion patterns of PCR amplification products with restriction endonuclease HpyCH4IV; e. Digestion patterns of PCR amplification products with restriction endonuclease BsaJI; f. Digestion patterns of PCR amplification products with restriction endonuclease BslI. Figure S2 Digestion patterns of PCR amplification products of SFBs with six different restriction endonucleases for “Taishanganying”. M. Ladder marker; 1–23. Digestion patterns of twenty-three independent positive clones. a. Digestion patterns of PCR amplification products with restriction endonuclease DpnII; b. Digestion patterns of PCR amplification products with restriction endonuclease Csp6I; c. Digestion patterns of PCR amplification products with restriction endonuclease HinfI; d. Digestion patterns of PCR amplification products with restriction endonuclease HpyCH4IV; e. Digestion patterns of PCR amplification products with restriction endonuclease BsaJI; f. Digestion patterns of PCR amplification products with restriction endonuclease BslI. Figure S3 PCR products of S-RNases amplified with primers Pru-C2 and Pa-C3R from genomic DNA of two parents (“Summit” and “Dabai”) and some of their cross-pollinated progeny. M. Ladder marker; S. “Summit”; T. “Dabai”; 1–44. Forty-four different individuals. a. Agarose gel electrophoresis patterns of S-RNase alleles; b. Polyacrylamide gel electrophoretic patterns of S-RNase alleles. Figure S4 PCR products of S-RNases amplified with primers Pru-C2 and Pa-C3R from genomic DNA of the parent (“Dabai”) and some of their self-pollinated progeny. M. Ladder marker; T. “Dabai”; 1–45. Forty-five different individuals. Figure S5 PCR products of S-RNases amplified with primers Pru-C2 and Pa-C3R from genomic DNA of two parents (“Summit” and “Taishanganying”) and some of their cross-pollinated progeny. M. Ladder marker; S. “Summit”; G. “Taishanganying”; 1–44. Forty-four different individuals. a. Agarose gel electrophoresis patterns of S-RNase alleles; b. Polyacrylamide gel electrophoretic patterns of S-RNase alleles. Figure S6 PCR products of S-RNases amplified with primers Pru-C2 and Pa-C3R from genomic DNA of parent (“Taishanganying”) and some of their self-pollinated progeny. M. Ladder marker; G. “Taishanganying”; 1–45. Forty-five different individuals. Figure S7 PCR products of SFBs amplified with primers PsSFB-F1 and PsSFB-R1 from genomic DNA of parent (“Dabai”) and some of their self-pollinated progenies. M. Ladder marker; T. “Dabai”; 1–45. Forty-five different individuals. Figure S8 PCR products of SFBs amplified with primers PsSFB-F1 and PsSFB-R1 from genomic DNA of two parents (“Summit” and “Dabai”) and some of their cross-pollinated progenies. M. Ladder marker; S. “Summit”; T. “Dabai”; 1–44. Forty-four different individuals. Figure S9 PCR products of SFBs amplified with primers PsSFB-F1 and PsSFB-R1 from genomic DNA of two parents (“Summit” and “Taishanganying”) and some of their cross-pollinated progenies. M. Ladder marker; S. “Summit”; G. “Taishanganying”; 1–21. Forty-four different individuals. Figure S10 PCR products of SFBs amplified with primers PsSFB-F1 and PsSFB-R1 from genomic DNA of parent (“Taishanganying”) and some of their self-pollinated progenies. M. Ladder marker; G. “Taishanganying”; 1–45. Forty-five different individuals. (DOC)