Transcriptome profiling of L02 cells upon Huh7-cell-derived large extracellular vesicles incubation

Analysis of the transcriptome of L02 cells upon Huh7-cell-derived large extracellular vesicles (lEVs) incubation. L02 cells were incubated with or without lEVs (10 μg/ml) derived from control or ACSL4 knocking down (ACSL4 KD) Huh7 cells for 3 days, and the gene expression profiling analysis were then performed. The result provides a new perspective on the crosstalk between Huh7 cells and L02 cells via lEV-mediated ACSL4 transmission. Total RNA was extracted from L02 cells by Trizol method and the gene expression was quantified by RNA-seq analysis.