TrakEm2 project files and corresponding electron microscopy files for "The architecture of cell differentiation in choanoflagellates and sponge choanocytes"

TrakEM2 project files and corresponding electron microscopy files
Transmission elelectron microscopy serial sections were imported as z-stacks into the Fiji plugin TrakEM2 and automatically aligned using default parameters,
except for increasing steps per octave scale to 5 and reducing maximal
alignment error to 50 px. Alignments were manually curated and adjusted if
deemed unsatisfactory. Organelles and subcellular compartments were manually
segmented and 3D reconstructed by automatically merging traced features along
the z-axis. Meshes were then preliminarily smoothed in TrakEM2.