In vivo single-cell ribosome profiling reveals cell-type-specific translational programs during aging. Duré et al.

Original images of Western blot images:

Replicate 1: used in Figure 4I
Sample loading scheme:
Lane 1 = Young Sca-1 pos; Lane 2 = Old Sca-1 pos; Lane 3 = Young Sca-1 neg; Lane 4 = Old Sca-1 neg; Lane 5 = Marker; Lane 6 = in vitro keratinocytes ctrl; Lane 7 = in vitrokeratinocytes STRESS
"Rep1_cJun_Chemi.tif" shows c-Jun protein bands and is used in the manuscript in Figure 4I. Image where the marker and membrane label "2", standing for "gel 2" is visible can be found in "Rep1_cJun_Chemi+Marker.jpg"
"Rep1_vinculin_Chemi.tif" is used in the manuscript in Figure 4I. Two membranes were imaged at the same time. Therefore a second images is provided, showing the gel/membrane numbering (pencil label "gel 1", "gel2"). The WB image used in Figure 4I originates from gel 2 (lower bands.)

Replicate 2: used for quantification in Figure S4L:
Loading scheme:
Lane 1 = Young Sca-1 pos; Lane 2 = Old Sca-1 pos; Lane 3 = Young Sca-1 neg; Lane 4 = Old Sca-1 neg; Lane 5 = Marker; Lane 6 = in vitro keratinocytes ctrl; Lane 7 = in vitro keratinocytes STRESS
"Rep2_cJun_Chemi.tif" shows c-Jun protein bands.
"Rep2_Vinc_Chemi.tif" shows Vinculin protein bands.
"Rep2_cJun_Chemi+Marker.jpg" shows cJun protein bands with markers.
"Rep2_Vinc_Chemi+Marker.jpg" shows Vinculin protein bands with markers.

Only tif images were used for quantification.