Aptamer-Based Targeting against Pseudomonas aeruginosa: A Non-SELEX Approach for Outer Membrane Destabilization Using Computational and Experimental Strategies

Antibiotic resistance is a growing threat to public health, especially from Pseudomonas aeruginosa, a superbug often found in hospitals. The research focuses on aptamers, tiny DNA molecules, that can weaken the bacteria’s protective outer layer, making it easier for antibiotics to reach their target. Using computer modelling, lab experiments, and infection models, the study identified aptamers that significantly enhance antibiotic effectiveness. This approach could lead to more effective treatments by supporting existing antibiotics, offering a new strategy in the fight against antimicrobial resistance.