Landscapes of TET1 and STAT5B binding on DNA across the entire genome in PDX2 B-ALL cells

Here we report both TET1-WT and catalytically dead mutant TET1 (TET1-MUT) directly interact with STAT5B in PDX2 B-ALL cells. To study the landscape of TET1 and STAT5B binding in whole genomic DNA and test if the STAT5B binding signal is affected by TET1, we conduct the ChIP-seq assay with flag antibody (TET1-WT, TET1-MUT) and STAT5B antibody (sgNS or sgTET1) respectively. The results showed that TET1 bound peaks overlap with STAT5B very well and the tag density of STAT5B on target genes decreases in TET1-impeded cells. We conclude that TET1 recruit STAT5B to the promoters of its target genes and further promote the target genes’ transcriptions. We generate the stable PDX2 cells with forced expression of flag-tagged TET1-CD-WT and TET1-CD-MUT. Meanwhile we also conduct the ChIP in wild type or TET1 knockout PDX2 cells(sgNS and sgTET1).