Thermal carryover effects of embryo incubation temperature through ontogeny in brook trout

We quantified possible thermal carryover effects in brook trout (Salvelinus fontinalis) across ontogeny and multiple genetically distinct wild and hatchery-origin populations. Populations were incubated in the laboratory in four differing thermal regimes: ambient stream-fed water, chronically warmed, ambient with a mid-winter cold-shock, and short-term warming. We examined upper thermal tolerance at the embryonic, fry (10 weeks post-full-hatch and 27-30 weeks post-full-hatch) and gravid adult life stages. In a separate experiment, we exposed developing embryos to seven-day heat stress events immediately following fertilization and at the eyed-egg-stage to examine possible thermal carryover effects at the 37-weeks-post-full-hatch stage.