3D multiplane dSTORM imaging of whole nuclear lamina

Raw data of single molecule photoswitching for 3D multiplane dSTORM of whole COS-7 nuclear lamina. Data from the first z plane, taken at time = 0 h, and the last z plane, taken at time = 1 h, are shown. Movies were captured at 50Hz under high laser power and in the presence of a reducing switching buffer.Also included is the complete SM coordinate list text file to reconstruct the 3D image of the whole COS-7 nuclear lamina.

本数据集收录了针对全COS-7细胞核纤层进行三维多平面dSTORM的单一分子光开关原始数据。展示了在时间点=0小时的数据（即首个z平面数据）以及在时间点=1小时的数据（即最后一个z平面数据）。所有影像均在50Hz的频率下，借助高激光功率以及还原型开关缓冲液的存在下捕获。此外，还包括了完整的SM坐标列表文本文件，以重建全COS-7细胞核纤层的3D图像。