Effect of beta3-adrenergic stimulation on liver gene expression in mice fed either a control diet or a western diet

The effect of beta3-adrenegic stimulation on liver gene expression was assessed in male mice after they had previously been fed a control diet or a western diet for 13 weeks. Mice fed the control diet were healthy. Mice fed the western diet became obese. Beta 3 adrenergic stimulation was achieved by using Beta 3 adrenergic receptor (CL316243). Overall, this experiment allowed us to investigate the effect of Beta3-adrenergic stimulation on liver gene expression in healthy mice and in obese mice. Thirty two 10-week-old mice were either fed a control diet (Ctrl; n= 16 mice) or a western diet (WD; n=16 mice) for 13 weeks. Control diet (Ctrl) contains 8.4% calories from fat, 19.3% calories from carbohydrates and 72.4% calories from proteins. Western diet (WD) contains 42% calories from fat, 42.7% calories from carbohydrates (mainly sucrose) and 15.2% calories from proteins and 0.2% cholesterol. Then, each group of these sixteen 23-week-old mice were fasted at ZT0, given CL316243 (3 mg/kg body weight; Sigma Aldrich) or vehicle (0.5% carboxymethylcellulose in sterilized water) by gavage at ZT10 and sacrificed at ZT16 (n = 8 per group).