Shallow shotgun sequencing of the microbiome recapitulates 16S amplicon results and provides functional insights

16S amplicon, deep shotgun metagenomic, and shallow shotgun metagenomic seqeunce characterization of the bacterial microbiome of feral horse fecal samples. Fresh, individual-linked fecal samples were collected from Sable Island feral horses (Sable Island, Nova Scotia) in 2014, during annual surveys of the horse population. Samples were kept on ice in the field and stored at -20C on Sable Island for two months, before being placed into longterm storage at -80C on the mainland.