File S1 - Ran GTPase-Activating Protein 1 Is a Therapeutic Target in Diffuse Large B-Cell Lymphoma

Supporting Materials and Methods.
Culturing DLBCL and B-lymphoblastoid cell lines. Proteomic analysis. Enzyme-linked immunosorbent assay (ELISA) for RanGAP1. Transfecting RANGAP1-specific shRNA into cell lines. Quantitative real-time polymerase chain reaction (Q-PCR). Figure S1. The standard curve of RanGAP1 serum level. Figure S2. Higher serum level of RanGAP1 in patients with high-grade BCL than in low-grade BCL and healthy controls. Figure S3. No cytotoxic effect on B or T cells from healthy donors at 48 hours. Figure S4. Double staining of RanGAP1 and Ki-67 in mantle cell and Hodgkin lymphomas. Table S1. Protein identification data expressed according to Paris guidelines.
(PDF)