FOXO1 enhances CAR T cell stemness, metabolic fitness and efficacy [human_FOXO1OE_stimulation/rest_RNAseq]

Investigating the transcriptional profile of human FOXO1 overexpressing α-lewisY CAR T cells after stimulation or after stimulation and rest Human anti-Lewis Y CAR T cells overexpressing FOXO1 or mCherry (control) were generated from healthy donor peripheral blood mononuclear cells (PBMC).Control or FOXO1- expressing CD8+ CAR T cells were assessed by RNA-Sequencing prior to stimulation, after 24 h activation with 0.1 μg/ml plate bound anti-Lewis Y or after 7 days recovery post stimulation with anti-Lewis Y.