File S1 - <i>Wolbachia</i> Divergence and the Evolution of Cytoplasmic Incompatibility in <i>Culex pipiens</i>

Supporting file contains Tables S1–S10. Table S1. Culex pipiens isofemale lines. Table S2. Reciprocal crosses between isofemale lines infected with wPip strains from the wPip-I group. (A) Reciprocal crosses between isofemale lines from La Réunion Island according to Atyame et al [17]. (B), Crosses between isofemale lines from Tunisia (Tn), Philippines (Ma-B), France (Bf-A), Grece (Ko), Spain (Ep-A and Ep-B) were performed in previous studies [16], [26] and Cotonou (Cot-A and Cot-B) were performed for this study. Crosses were classified either compatible (C, hatching rate (HR) >90%) or incompatible (IC, HR = 0%, shaded). The number of egg-rafts collected in each cross is bracketed. Note that crosses between mosquitoes from the same isofemale line are always compatible. Table S3. Reciprocal crosses between isofemale lines infected with wPip strains from the wPip-II group. Isofemale lines were isolated from samples collected in France (Lv), Brisbane (Au) and Cyprus (Ke-A and Ke-B). All crosses were performed by Duron et al. [16]. C = compatible crosses (HR >90%). The number of egg-rafts collected in each cross is bracketed. Table S4. Reciprocal crosses between isofemale lines infected with wPip strains from the wPip-III group. Isofemale lines were isolated from samples collected in California (Sl and Mc), New Mexico (Albu-3) and France (Bf-B, Trio-2 and Trio-7). *,Crosses corresponding to data from Duron et al. [16]. Crosses were classified either compatible (C, for HR >90% or incompatible (IC, HR = 0%, shaded). The number of egg-rafts collected in each cross is bracketed. Table S5. Reciprocal crosses between isofemale lines infected with wPip strains from the wPip-IV group. (A and B), reciprocal crosses between the isofemale line Is (from Turkey) and the isofemale lines from Tunisia (Bou-1, Bou-2, Kef-1, Kef-2, Tab-1, Tab-2), from Algeria (Dou-1, Dou-2, Guel-1, Guel-2, Kal-1, Kal-2, Lac-1, Lac-2, Souk-2, Ha) and from Italy (CAA). (C), reciprocal crosses between the isofemale lines from Tunisia. C = compatible crosses (HR >90%). The number of egg-rafts collected in each cross is bracketed. Table S6. Reciprocal crosses between isofemale lines infected with wPip strains from the wPip-V group. These crosses correspond to data from Duron et al. [16] and isofemale lines were from China (Kara-C) and Philippines (Ma-A). C = compatible crosses (HR >90%). The number of egg-rafts collected in each cross is bracketed. Table S7. Crossing relationships between mosquito lines infected with wPip strains from the wPip-I group and lines infected with strains from wPip-II, wPip-III, wPip-IV and wPip-V groups. (A) Between wPip-I infected males and females infected with wPip-II, wPip-III, wPip-IV and wPip-V groups. (B) Between wPip-I infected females and males infected with wPip-II, wPip-III, wPip-IV and wPip-V groups. *, Crosses corresponding to data from Duron et al. [16], [26]. Crosses were classified either compatible (C, HR >90%) or incompatible (IC, HR = 0%, shaded). Bi-directionally incompatible crosses are underlined. The number of egg-rafts collected in each cross is bracketed. Table S8. Crossing relationships between mosquito lines infected with wPip strains from the wPip-II group and lines infected with strains from wPip-III, wPip-IV and wPip-V groups. (A) Between wPip-II infected males and females infected with wPip-III, wPip-IV and wPip-V groups. (B) Between wPip-II infected females and males infected with wPip-III, wPip-IV and wPip-V groups. *, Crosses corresponding to data from Duron et al. [16], [26]. Crosses were classified either compatible (C, HR >90%) or incompatible (IC, HR = 0%, shaded). Bi-directionally incompatible crosses are underlined. The number of egg-rafts collected in each cross is bracketed. Table S9. Crossing relationships between mosquito lines infected with wPip strains from the wPip-III group and lines infected with strains from wPip-IV and wPip-V groups. (A) Between wPip-III infected males and females infected with wPip-IV and wPip-V groups. (B) Between wPip-III infected females and males infected with wPip-IV and wPip-V groups. *, Crosses corresponding to data from Duron et al. [16]. Crosses were classified either compatible (C, HR >90%) or incompatible (IC, HR = 0%, shaded). Bi-directionally incompatible crosses are underlined. The number of egg-rafts collected in each cross is bracketed. Table S10. Crossing relationships between mosquito lines infected with wPip strains from the groups wPip-IV and wPip-V. *, Crosses corresponding to data from Duron et al. [16]. IC = incompatible crosses (HR = 0%, shaded). Bi-directionally incompatible crosses are underlined. The number of egg-rafts collected in each cross is bracketed. (PDF)