Expression data from Jaridon 6 on the human esophageal cancer cells

Jaridon 6, an ent-kaurene diterpenoid derivative from Rabdosia rubescens (Hemsl.) Hara, possesses strong antitumor activity in esophageal cancer cells. This study explored the underlying molecular events of Jaridon 6’s anti-tumor activity in esophageal cancer cells through the cDNA microarray. We wanted to obtain early changes of RNA by Jaridon 6's action on the esophageal cancer 109 cells, especially in early apoptosis stage. The IC50 of Jaridon 6 is 15 µM. When the esophageal cancer 109 cells were acted by 15 µM Jaridon 6 for 16h, the apoptosis rate is approximately 40%, near to the early apoptosis stage. So the esophageal cancer 109 cells were treated with 15 µM Jaridon 6 for 16 h for RNA extraction and hybridization on Affymetrix microarrays.