DYNLL1 targeting and local nuclear alignment in U2OS with a telomeric 12q regional pattern — reproducible data and analysis

Reproducible data and analysis for a descriptive study of local nuclear alignment after DYNLL1 targeting in U2OS cells, alongside a telomeric 12q regional pattern. The primary analysis contains 7,875 native DNA fields, 875 wells, 67 selected genes and 335 gene/date contrasts across five acquisition dates. Dates reuse one reagent pool per gene and shared controls; independent targeting, rescue and the molecular mechanism remain unvalidated. The archive retains complete core focal/field inputs, all alignment definitions and sensitivity analyses, grouped conventional-profile predictions, the plate/rank audit, same-plate comparisons, all 88 figure-panel sources, 22 supplementary tables and an explicit 25-reference verification matrix. All selected genes, dates and nonconfirming results are retained. Editorial drafts and private review correspondence are excluded. Bulk raw JUMP images and the Cellpose-SAM checkpoint are linked by public URLs and hashes rather than bundled in full. Code mirror: https://github.com/subhajit-dutta-bio/dynll1-u2os-nuclear-alignment . Original analysis code is MIT licensed; original derived release outputs are CC BY 4.0. Upstream data and metadata retain their own terms, as described in THIRD_PARTY_NOTICES.txt. This is a data and code deposit, not a journal submission, wet-lab validation, or claim of acceptance.