Transcript assembly of plastome replication transcripts in sugarcane and plastome targeted single strand DNA binding proteins in Azolla used as the basis for sequencing of said proteins in Sugarcane N22 cultivar and Azolla filiculoides. lastome replication transcripts in sugarcane and plastome targeted single strand DNA binding proteins in Azolla.

Transcript replication proteins are critical for plastome replication in all plants, however there has been no large-scale analysis in grasses. High depth transcriptome datasets were used to assemble these proteins in sugarcane cultivar SP80-3280. Primers were designed and the corresponding transcripts were sequenced with MinION in sugarcane cultivar N22. For the phylogeny of single strand DNA binding proteins the transcripts were assembled in Azolla filiculoides prior to sequencing.