RNA-sequencing in two MYCN amplified/high-risk and one non-MYCN amplified/low-risk neuroblastoma cell lines

RNA-sequencing has been used to obtain and observe transcriptome-wide differences that occur in two parasspeckle-deficient, MYCN-amplified/model high-risk and one paraspeckle-abundant, non-MYCN-amplified/low-risk cell lines, to decipher the role of paraspeckles in this cancer type. Overall design: Methods: Cell lines were grown without any treatment for 48 hours, after which cells were lysed. RNA-seq was conducted to show transcriptome wide differences occurring between low and high risk neuroblastoma cell lines. Our study has shown differential NEAT1_1 and NEAT1_2 expression levels in low versus high risk neuroblastoma cell lines.