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RNA-seq analysis of human embryonic stem cell-derived enterocytes

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA649090
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To develop intestinal cells derived from human embryonic stem cells as small intestine models, the differentiated cells were sequenced. To perform RNA-seq, total RNA was extracted from the cells. cDNA libraries were generated according to standard procedures using the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit (Vienna, Austria) and the libraries were sequenced on an Illumina NextSeq500 (San Diego, CA, USA) in single-end (SE) 75-base mode.
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2020-07-28
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