Investigate the effect of MSCs treatment on the glomeruli of mice with lupus nephritis by RNA sequencing

Human umbilical cord mesenchymal stem cells (hUC-MSCs) have shown potential as a therapeutic option for lupus nephritis (LN), particularly in patients refractory to conventional treatments. Despite extensive translational research on MSCs, the precise mechanisms by which MSCs migrate to the kidney and restore renal function remain incompletely understood. Here, we aim to clarify the spatiotemporal characteristics of hUC-MSC migration into LN kidneys and their interactions with host cells in microenvironment. This study provides valuable insights into the significant involvement of CXCL10-CXCR3 axis in hUC-MSC migration to the LN kidneys and the subsequent remodeling of renal immune microenvironment. We set up a blank control group of mice, a lupus nephritis model group of mice (MRL/lpr mice), and MRL/lpr mice treated with MSCs, and then isolated the mice's glomeruli and performed single-cell sequencing.