Expression data from V156D matn1 knock-in mouse rib chondrocytes at 5 days

V156D matn1 mutation (homologous to the V194D matn3 mutation causing Multiple Epiphyseal Dysplasia (MED)) was introduced by gene targetting in order to evaluate the potential of matrilin-1 to be a novel chondrodysplasia based on domain homology and aminoacid conservation. We used microarrays to analyse the differential expression of genes between wild type and mutant animals at day 5 (postnatal) Rib chondrocyes were extracted from 5 littermates of known genotype and RNA was extracted from the pooled smaple using the TriZOL method; the RNA integrity was checked using BioAnalyser, and hybridised to the Affymetrix Mouse Genome 430 2 Array