X-linking in the asymmetric ATP-bound state.

NOTE: In Tables 2 and 3 the distance between Cα atoms has been estimated as follows: 2× (length of cysteine side chain + distance to spacer) + length of spacer.aPrecise reducing agent not provided. Reducing agents used in this study were N,N’-o-phenylenedimaleimide, N,N’-p-phenylenedimaleimide and 1,6-bismaleimidohexane. The spacer span lengths range from ∼0.3 to ∼1.0 nm.bCross-linking was attempted with each of the following spacers: M2M (approximate cross-linking span: 0.52 nm), M3M (∼0.65 nm), M4M (∼0.78 nm), M5M (∼0.91 nm), M6M (∼1.04 nm), M8M (∼1.30 nm), M11M (∼1.69 nm), M14M (∼2.08 nm) and M17M (∼2.47 nm). The distance range given is calculated from the span of the largest and smallest spacer cross-linked.cReducing agent is copper phenanthroline. Distance between the S atoms is ∼0.37 nm.