Gene expression in the hTSCs of si-NC and si-PR-SET7 KD hTSCs

To detect the gene profiles in si-NC and si-PR-SET7 KD hTSCs, hTSCs are collected and subjected to RNA-Seq. After aligned to mouse hg38 by HISAT2, RPKM value was calculated by Edger. Our results show that PR-SET7 as the key regulator for hTSCs. There are also some genes differentially expressed after PR-SET7 KD, some of the DEGs were further confirmed by qPCR, the DEGs were associated with viral mimic inflammatory activities, antiviral innate immune response, genomic instability, and programmed cell death, indicating the essential role of PR-SET7. This RNA-Seq data provides fundamental information for our further physiological study of PR-SET7. Total RNAs in hTSCs from two independent experiments were extracted by TRIzol and subjected to RNA-Seq by MGISEQ 2000. The expression of level of each gene is normalized to RPKM.