FRET efficiency for Epac sensors determined by frequency-domain FLIM.
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Measured fluorescence lifetimes and calculated FRET efficiencies for the different Epac-constructs before (−) and after (+) stimulation with IBMX and Forskolin for 10 min.1n, number of cells;2τφ average phase lifetime ± s.d.;3τM, average modulation lifetime ± s.d.;4E, average FRET efficiency calculated from τφ or τM according to (1- τDA/τD) * 100%, using τD values of 3.71 ns and 3.80 ns for mTurquoise and τD values of 2.28 ns and 2.99 ns for mECFP, for phase and modulation lifetimes respectively [9].
创建时间：
2015-12-02
