NeoPPI - BRAF

The protein-protein interactions of BRAF-V600E or BRAF wild type detected using the NanoLuc luciferase-based bioluminescence resonance energy transfer (BRETn) technology-based quantitative high-throughput differential screening (qHT-dS) platform. The qHT-dS allows comparative screening of wild-type and mutant allele counterparts for the detection of differential interactions with cancer-associated proteins in live mammalian cells.
To differentiate gain of interactions (Go-PPI) from loss of interactions (Lo-PPI), the difference between WT and MUT PPI curves were calculated to obtain differential scores (differential_score), corresponding p-values (pvalue_ds), and p-values adjusted for multiple comparisons (qvalue_ds).
The statistically significant edges (ss-Go-PPI, light blue) were defined based on the differential_score > 1 and pvalue_ds < 0.01. For ss-Lo-PPI edges (light red) the differential_score < 1 and pvalue_ds < 0.01.

The high-stringency nedges (hs-Go-PPI, blue or hs-Lo-PPI, red) include the statistically significant PPIs for which qvalue_ds < 0.01, and differential_score ≥ 1.5 or ≤ 1/1.5, respectively.

The hs-Go-PPI edges that were further confirmed in a single-lowest-dose assay were prioritized as high-confidence edges (hc-Go-PPI, dark blue).