Analysis of mRNA and miRNA expression in mouse monocyte macrophage infected with Salmonella enterica subsp. entericaserovar Dublin

Salmonella enterica subsp. entericaserovar Dublin (Salmonella Dublin), as a potential zoonotic pathogen, can cause a variety of diseases in humans and animals. Clinical symptoms include gastroenteritis, sepsis, and local infection. In addition, these bacteria can survive, proliferate, and spread in macrophages, seriously threatening the development of animal husbandry and human health. At present, miRNAs have received extensive attention as major biomarkers in various diseases. In this study, we used high-throughput sequencing technology to analyze the differentially expression (DE) of mRNAs and miRNAs in mouse monocyte macrophage cells(RAW264.7) infected with Salmonella Dublin, and the regulatory networks of miRNAs and mRNAs were analyzed. A total of 1080 DE mRNAs were screened, corresponding to 492 up-regulated and 588 down-regulated genes. In addition, DE miRNA analysis showed that 123 DE miRNAs were screened, including 66 up-regulated and 57 down-regulated miRNAs. GO functional annotation showed significant enrichment in functions such as metabolic processes, cytoplasm, and catalytic activity. In KEGG enrichment analysis, DE miRNAs were mainly enriched in multiple pathways related to macrophage immune response, such as material metabolism, MAPK, and apoptosis. At the same time, we constructed a mRNA-miRNA regulatory network and verified the accuracy of DE mRNA and DE miRNA sequencing results by RT-qPCR. The above results provide experimental data to support the revelation of host regulatory mechanisms during Salmonella Dublin infection and the development of new detection methods and therapeutic drugs.