Comparing the gene signature of mouse primary keratinocytes expressing a KrasG12D allele on mixed C57BL/6 :FVB/N background versus an F3 FVB/N backcross

LSL-KrasG12D mice were backcrossed with FVB/N mice and primary keratinocytes were harvested were from F1 and F3 and transduced in vitro with a Cre adenovirus to express a single mutant Kras allele. When cultures were harvested and transplanted on athymic hosts large squamous papilloma formed within 3 weeks but only from F3 keratinocyte cultures To further characterize what unique transcriptional changes could be attributed to tumorigenic (F3 backcross) versus non tumorigenic (F1) cultures, we subjected F1+Cre and F3 + Cre KrasG12D keratinocyte cultures to RNA-seq analysis.