Enterococcus faecium Whole Genome sequencing

The genome of Enterococcus faecium was assembled de novo using SPAdes, with sequencing reads polished to ensure high accuracy. Annotation of the genome was performed using Prokka, providing detailed information about coding sequences, rRNAs, tRNAs, and other genomic features. Functional annotation of the gene IDs was enhanced using Diamond and UniProt databases to identify genes and assign their biological roles.The study also included predictions of antimicrobial resistance (AMR) genes using tools such as ResFinder, NDARO, PATRIC and CARD (Comprehensive Antibiotic Resistance Database), and pathogenicity factors were analyzed using PATRIC_VF, VFDB, and Victors. This comprehensive analysis provides insights into the genomic characteristics, resistance mechanisms, and pathogenic potential of E. faecium, a microorganism of significant clinical and environmental relevance.