Specific binding energies and information contents.

‘’ is the specific binding energy of the highest affinity site as determined by the intercept of the regression lines in Figure 6. ‘’ is the information content in bits of the corresponding energy logo for each mutant over the range of to (Figure 5). ‘’ is the information content of the in vivo binding selection logo over the range of to (Figure 7). * denotes that we are not confident in the calculation for that mutant.