Codon usage bias characteristic analysis of chloroplast Genomes in X Triticosecale sp., Triticum monococcum, Triticum turgidum, Triticum anstivum, and Secale cereale

The datasets included five species' sequences, which were selected from complete chloroplast genome sequences. The complete chloroplast genome sequences of the five species were downloaded from the NCBI database (https://www.ncbi.nlm.nih.gov). For X Triticosecale sp. (GenBank accession number: ON422331.1), 87 sequences were obtained; for Triticum monococcum (NC_021760.1), 79 sequences; for Triticum turgidum (NC_024814.1), 82 sequences; for Triticumiv aestum (KC912694.1), 79 sequences; and for Secale cereale (JF829824.1), 77 sequences. After filtering out sequences shorter than 300 bp, removing duplicates, excluding sequences without ATG as the start codon, and eliminating sequences with abnormal termination codons or internal termination codons within the CDS, the final datasets comprised 52, 54, 49, 52, and 51 sequences, respectively. These datasets could be used to analyze codon usage bias.