Analysis of the small RNA profile in heso1 urt1 ski2 in Arabidopsis with biological replications

Non-templated uridine addition at the 3' ends of RNAs (RNA uridylation) is a widespread post-transcriptional modification of eukaryotic RNAs. Uridylation regulates diverse RNA metabolism processes including maturation, localization and stabilization. To date, the primary function of RNA uridylation is to promote RNA turn-over. Here we demonstrate that HESO1, URT1 and SKI2 redundantly regulate Arabidopsis leaf chlorophyll content. This phenotype was mainly caused by disturbance of mRNA degradation and spurious siRNA biogenesis from Transketolase 1 (TKL1) locusand was substantially suppressed by the rdr6. sRNA-seq approach was used to investigate the physiological relevance veiled by the developmental phenotypes in Ler, ski2_6 (s), heso1_2 urt1_3 (hu), heso1_2 urt1_3 ski2_6 (hus) and heso1_2 urt1_3 ski2_6 rdr6_8 (husr). A number of endogenous genes, including many miRNA target genes, manifest transitive 21-22 nt siRNA production and compromised gene expression in hus in an RDR6-dependent manner. Taken together, our study highlights the significance of mRNA uridylation for plant development. In this experiment, we did three replicates in sequencing that help increase the power in identify the differentially represented genes.