ChIP-seq analysis about Dot1l and Npm1 samples

To identify whether Dot1l or Npm1 were enriched in MERVL directly, we performed Dot1l and Npm1 ChIP-seq in wildtype ESCs. We conclude that Dot1l and Npm1 binding were enriched on MERVL-int region, suggesting a direct regulatory role of Dot1l and Npm1 on MERVL expression. Overall design: We carried out Dot1l and Npm1 ChIP-seq with anti-Dot1l, anti-Npm1 antibodies in wildtype ESCs in order to identify whether Dot1l or Npm1 were enriched in MERVL.