Gene expression profile of primary CLL cells transduced with a mutated form on NOTCH1 following IgM stimulation

In CLL, NOTCH1 is the most commonly mutated gene at diagnosis and it is associated with a poor outcome. The mechanisms underlying how NOTCH1 contributes to disease progression, resistance to treatment and Richter’s transformation remain largely unknown. Data suggests a link between BCR activation and NOTCH1 signalling so the main goal of this study is to compare the gene expression profile of primary CLL cells transduced with the intracellular part of NOTCH1 lacking of the PEST domain vs cells transduced with an empty vector following IgM stimulation. Primary CLL cells were isolated through cell sorting 4 days post transusction with a NOTCH1ΔPEST or an empty retroviral vector. The cells were then stimulated with a F(ab')2 Anti-human IgM for 48 hours