Expression profilling of C. glabrata response to fluconazole and the role of the transcription factor Mar1 in such response

The goals of this study are to compare C. glabrata transcriptome profiling (RNA-seq) upon exposure to the antifungal fluconazole in order to assess antifungal response. The role of the transcription factor Mar1 is clarified through comparison of the transcrptome profiles of WT and Mar1 mutant cells. mRNA profiles of WT and ?mar1 were generated by deep sequencing, in duplicate, using Illumina HiSeq. The sequence reads that passed quality filters were analyzed with TopHat followed by HTSeq. Overall design: Yeast mRNA profiles after 1h fluconazole exposure of WT and ?mar1 cells were generated by RNA-Seq, in duplicate (each duplicate made of triplicate samples pooled together) using Illumina HiSeq