Single-cell Transcriptome of Bronchoalveolar Lavage Fluid Reveals Dynamic Change of Macrophages During SARS-CoV-2 Infection in Ferrets

Single-cell transcriptome of bronchoalveolar lavage fluid cells longitudinally obtained from SARS-CoV-2-infected ferrets was analyzed. Changes in cell proportions and characteristics in uninfected control, at 2 days post-infection (dpi), and 5 dpi were noted. Macrophages were classified into the subpopulations with dynamic changes quantitatively and qualitatively over time. Fundamental aspects of the immune response dynamics provoked by SARS-CoV-2 infection were highlighted in this study. Overall design: Total 10 single cell RNA seq libraries (10X Chromium, v3) were sequenced. All samples are bronchoalveolar lavage fluid cells from ferret. Samples are consist with 3 control (healthy) ferrets, 3 ferrets at 2 dpi of SARS-CoV-2, and 4 ferrets of 5 dpi.