Proteomic analysis results of platelet-rich fibrin extract

Dataset title

Proteomic analysis of platelet-rich fibrin extract activated by cyclic low-frequency ultrasound

Dataset overview

This dataset describes the proteomic characterization of platelet-rich fibrin extract (PRFe) generated by cyclic low-frequency ultrasound (CLFU)–mediated activation of platelet-rich fibrin (PRF). PRF was prepared from peripheral venous blood collected from healthy adult donors and activated using a biomimetic mechanical stimulation protocol to regulate platelet degranulation and protein release. The resulting PRFe was analyzed by mass spectrometry–based proteomics to define its protein composition.

Sample preparation

PRF clots were obtained from freshly collected human peripheral blood without anticoagulants. For PRFe preparation, PRF samples were immersed in saline and activated using an intermittent CLFU protocol. After activation, PRFe was collected, centrifuged to remove residual fibrin components, and filtered to obtain clarified protein extracts suitable for proteomic analysis.

Proteomic analysis

Protein composition of PRFe was analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Samples were processed using standard proteomics workflows, including protein digestion and peptide purification. Data were acquired on a high-resolution LC–MS/MS platform. Protein identification and quantification were performed using established bioinformatics pipelines, and relative protein abundance was estimated using intensity-based absolute quantification (iBAQ).

Data content

The dataset includes raw mass spectrometry files and processed protein identification and quantification results. More than 300 proteins were identified in PRFe samples, mainly comprising plasma- and platelet-associated proteins such as albumin, transferrin, apolipoproteins, immunoglobulin chains, and other blood-derived components, reflecting the autologous origin of PRFe.

Functional annotation

Functional annotation and enrichment analyses were conducted using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Identified proteins were associated with biological processes related to complement activation, immune regulation, angiogenesis, extracellular matrix organization, and wound-related responses. Cellular component analysis showed enrichment in blood microparticles, platelet α-granules, and extracellular matrix–associated compartments. KEGG pathway analysis highlighted complement and coagulation cascades and extracellular matrix–receptor interaction pathways.

Data availability and reuse

This dataset provides a qualitative overview of the protein composition of PRFe generated under a standardized, mechanically programmed activation protocol. The data can be reused for comparative studies of platelet-derived biomaterials, extracellular matrix–associated signaling, and proteomics-based research related to tissue regeneration and angiogenesis.