Tn-seq analysis of genetic interactions with Acinetobacter baumannii YkuD-family cell wall proteins

The goal of this study is to elucidate the functions of proteins involved in modulating the cell wall of the pathogen Acinetobacter baumannii. The pathways that become required for growth in the absence of each protein were identified to identify leads on their function. This was achieved by Tn-seq analysis of dense mariner transposon banks constructed in ElsL-and Ldt- mutant strains of ATCC 17978. Knowledge of the function of these proteins can facilitate development of improved strategies for treatment of A. baumannii infections.