Differential expression of circRNAs in virus infection

There is increasing evidence showing that aberrant expressions of circRNAs are implicated in various physiological or pathological processes. Yet, their roles in antiviral innate immunity remain unknown. To reveal the implications of circRNAs in antiviral innate immunity, we used Human circRNA Array v2.0 microarrays to measure the expression profiles of circRNAs in HEK293 cells infected with or without Sendai virus(SeV). We found 82 up-regulated and 20 down-regulated circRNAs(fold change >2; P <0.05) in HEK293 cells after SeV infection. Upregulation of circRNA hsa_circ_0006916 was established using Real-time quantitative PCR. These results showed abrrant expression profiles of circRNAs in HEK293 cells after SeV infection and revealed their potential roles in antiviral innate immunity. In this study, we determined expression profile of circRNAs in HEK293 cells infected with sendai virus (T293_1P, T293_2P, T293_3P) and without sendai virus (T293_1_, T293_2_, T293_3_) using Human circRNA Array v2.0 microarray (8×15K, Arraystar).