三十五份吉林省猴头菇菌株的全基因组序列分析

Currently, research on regional genetic diversity and population genetic structure of Hericium erinaceus remains relatively scarce. Additionally, the analysis of the genetic background of germplasm resources has largely relied on fragmented molecular markers, lacking a comprehensive landscape of genome-wide variations, and thereby hindering the progress of genetic improvement. In this study, whole-genome resequencing of 35 H. erinaceus germplasm resources from Jilin Province was performed. Based on variation data such as single nucleotide polymorphisms (SNPs) and small insertions/deletions (InDels), their genetic structure, evolutionary relationships, and diversity characteristics were systematically analyzed. Population genetic structure analysis revealed that the optimal number of clusters was K=2, with significant genetic isolation between the cultivated strains and wild populations (Fst=0.079 8-0.090 4), indicating that artificial selection has led to a significant deviation in the genomic genetic background of the cultivated populations from wild populations. Genetic distance and linkage disequilibrium (LD) analyses showed low genetic differentiation among wild populations (Fst=0.022 5- 0.030 5) with an LD decay distance of 6-15 kb (r2H. erinaceus germplasm resources. By deciphering genetic patterns, it aids in broadening the genetic basis of cultivated populations and promotes sustainable industrial development.