Gene Editing of Anp32a Confers Resistance to Avian Influenza in Chicken

Comparison of transcriptomic profiles of primordial germ cells and derived fibroblast cell lines from homozygous, CRISPR/Cas9 gene-edited, ANP32A knockout and mutant chickens Overall design: We used CRISPR/Cas9 to perform precise sequence changes in ANP32A to generate genome-edited PGC cell lines, which were also differentiated into fibroblast-like cell lines, all of which were subjected to RNA-seq in their native state.